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1996-03-09
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Document 0155
DOCN M9650155
TI Nonisotopic hybridization assay for determination of relative amounts of
genotypic human immunodeficiency virus type 1 zidovudine resistance.
DT 9605
AU Eastman PS; Boyer E; Mole L; Kolberg J; Urdea M; Holodniy M; Chiron
Corporation, Emeryville, California 94608, USA.
SO J Clin Microbiol. 1995 Oct;33(10):2777-80. Unique Identifier : AIDSLINE
MED/96087230
AB A nonisotopic hybridization assay for human immunodeficiency virus
genotypic zidovudine resistance determination is described. Biotinylated
PCR product was hybridized with enzyme-labeled probes for wild-type or
resistant mutant sequences and detected colorimetrically or
chemiluminescently in a microplate format. Changes in
mutant-to-wild-type ratios allow for the monitoring of longitudinal
patient samples.
DE Antiviral Agents/*PHARMACOLOGY Chemiluminescence Drug Resistance,
Microbial/GENETICS Genotype Human HIV Infections/BLOOD HIV-1/*DRUG
EFFECTS/GENETICS Mutation Nucleic Acid Hybridization Polymerase Chain
Reaction Reverse Transcriptase Inhibitors/*PHARMACOLOGY RNA-Directed
DNA Polymerase/*GENETICS Sensitivity and Specificity Support, Non-U.S.
Gov't Zidovudine/*PHARMACOLOGY JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).